84-Well Tray Scientific Principle

The Colony Counting Test Reagent uses enzyme-substrate technology to detect bacterial colony counts in water. It contains different enzyme substrates designed for different bacterial enzymes. When bacterial enzymes break down the substrates, fluorescent groups are released. Determine the total colony count by viewing fluorescent wells under a 365 nm or 366 nm UV lamp and consulting the MPN table.

Operation steps

1. Add 1 mL of water sample and 9 mL of sterile water to the brown bottle containing solid reagent. Shake until fully dissolved.
2. Pour the solution into the center of the assay plate.
3. Rotate the assay disk so the sample fills the well grid.
4. After the wells are filled, tilt the disk so excess liquid is absorbed by the sponge strip.
5. Invert the assay disk and incubate at 36 ±1°C for 48 hours.
6. Count fluorescent wells under a UV lamp and compare the result with the MPN table.

For sample volumes of 0.1 mL, 0.01 mL or less, dilute the sample and add it for testing.